Bovine GM-CSF: Molecular cloning and biological activity of the recombinant protein
Identifieur interne : 003218 ( Main/Exploration ); précédent : 003217; suivant : 003219Bovine GM-CSF: Molecular cloning and biological activity of the recombinant protein
Auteurs : Charles R. Maliszewski [États-Unis] ; Michael A. Schoenborn [États-Unis] ; Douglas Pat Cerretti [États-Unis] ; Janis M. Wignall [États-Unis] ; Kathleen S. Picha [États-Unis] ; David Cosman [États-Unis] ; Robert J. Tushinski [États-Unis] ; Steven Gillis [États-Unis] ; Paul E. Baker [États-Unis]Source :
- Molecular Immunology [ 0161-5890 ] ; 1988.
English descriptors
- Teeft :
- Amino, Amino acid sequence, Amino acid sequence homology, Amino acid sequences, Amino acid signal sequence, Amino acids, Amino terminus, Assay, Attta sequence motif, Biological activity, Bone marrow cells, Bovine, Bovine assay, Bovine bone marrow proliferation assay, Bovine cdna, Bovine cdna clone, Bovine genomic, Bovine probe, Cdna, Cdna library, Cell line, Coding region, Cosman, Culture supernatants, Gasson, Genomic, Grabstein, Granulocyte, Human cdna probe, Human factor, Human probe, Infectious agents, Mammalian expression vector, Marrow, Mature cells, Mature protein, Metcalf, Mrna, Murine, Northern blot analysis, Nucleic acids, Nucleotide, Nucleotide sequence, Phorbol myristate acetate, Plasmid, Potential sites, Previous studies, Recombinant, Recombinant murine, Restriction endonucleases, Signal sequences, Southern blot, Species specificity, Supernatant, Transfected, Wong.
Abstract
Abstract: The lymphokine granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates the growth and differentiation of granulocytes and macrophages from bone marrow progenitors, and regulates biological functions expressed by mature cells of these lineages. In order to isolate a bovine GM-CSF cDNA, a cDNA library, generated from the BT2 bovine T cell line, was screened with a human GM-CSF cDNA probe. A cDNA clone was isolated with an insert of 783 bp, that would encode a protein of 143 amino acids, with a predicted mol. wt of 16,160. Bovine GM-CSF exhibits a high degree of sequence homology with mouse and human GM-CSF at both the nucleotide and amino acid levels. Comparison of GM-CSF amino acid sequences from the three species indicates that the bovine GM-CSF precursor contains a putative 17 amino acid signal sequence, cleavage of which would yield a 14,250 mol. wt protein. The cDNA was inserted into a mammalian expression vector and transfected into COS-7 monkey kidney cells. Biologically active bovine GM-CSF was secreted as judged by a bovine bone marrow proliferation assay. Bovine GM-CSF was weakly active in both human and mouse bone marrow proliferation assays. In contrast, human GM-CSF was weakly active on bovine but not murine mouse bone marrow cells and mouse GM-CSF was only active on murine bone marrow cells.
Url:
DOI: 10.1016/0161-5890(88)90120-4
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002B08
- to stream Istex, to step Curation: 002B08
- to stream Istex, to step Checkpoint: 001F80
- to stream Main, to step Merge: 003289
- to stream Main, to step Curation: 003218
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Bovine GM-CSF: Molecular cloning and biological activity of the recombinant protein</title><author><name sortKey="Maliszewski, Charles R" sort="Maliszewski, Charles R" uniqKey="Maliszewski C" first="Charles R." last="Maliszewski">Charles R. Maliszewski</name></author><author><name sortKey="Schoenborn, Michael A" sort="Schoenborn, Michael A" uniqKey="Schoenborn M" first="Michael A." last="Schoenborn">Michael A. Schoenborn</name></author><author><name sortKey="Cerretti, Douglas Pat" sort="Cerretti, Douglas Pat" uniqKey="Cerretti D" first="Douglas Pat" last="Cerretti">Douglas Pat Cerretti</name></author><author><name sortKey="Wignall, Janis M" sort="Wignall, Janis M" uniqKey="Wignall J" first="Janis M." last="Wignall">Janis M. Wignall</name></author><author><name sortKey="Picha, Kathleen S" sort="Picha, Kathleen S" uniqKey="Picha K" first="Kathleen S." last="Picha">Kathleen S. Picha</name></author><author><name sortKey="Cosman, David" sort="Cosman, David" uniqKey="Cosman D" first="David" last="Cosman">David Cosman</name></author><author><name sortKey="Tushinski, Robert J" sort="Tushinski, Robert J" uniqKey="Tushinski R" first="Robert J." last="Tushinski">Robert J. Tushinski</name></author><author><name sortKey="Gillis, Steven" sort="Gillis, Steven" uniqKey="Gillis S" first="Steven" last="Gillis">Steven Gillis</name></author><author><name sortKey="Baker, Paul E" sort="Baker, Paul E" uniqKey="Baker P" first="Paul E." last="Baker">Paul E. Baker</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:658059190C2A84658067CF4EECD00BE248EE47DE</idno><date when="1988" year="1988">1988</date><idno type="doi">10.1016/0161-5890(88)90120-4</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-8FQZXXQX-K/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002B08</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002B08</idno><idno type="wicri:Area/Istex/Curation">002B08</idno><idno type="wicri:Area/Istex/Checkpoint">001F80</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001F80</idno><idno type="wicri:doubleKey">0161-5890:1988:Maliszewski C:bovine:gm:csf</idno><idno type="wicri:Area/Main/Merge">003289</idno><idno type="wicri:Area/Main/Curation">003218</idno><idno type="wicri:Area/Main/Exploration">003218</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Bovine GM-CSF: Molecular cloning and biological activity of the recombinant protein</title><author><name sortKey="Maliszewski, Charles R" sort="Maliszewski, Charles R" uniqKey="Maliszewski C" first="Charles R." last="Maliszewski">Charles R. Maliszewski</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Immunex Corporation, Seattle, WA 98101</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Schoenborn, Michael A" sort="Schoenborn, Michael A" uniqKey="Schoenborn M" first="Michael A." last="Schoenborn">Michael A. Schoenborn</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Immunex Corporation, Seattle, WA 98101</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Cerretti, Douglas Pat" sort="Cerretti, Douglas Pat" uniqKey="Cerretti D" first="Douglas Pat" last="Cerretti">Douglas Pat Cerretti</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Immunex Corporation, Seattle, WA 98101</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Wignall, Janis M" sort="Wignall, Janis M" uniqKey="Wignall J" first="Janis M." last="Wignall">Janis M. Wignall</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Immunex Corporation, Seattle, WA 98101</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Picha, Kathleen S" sort="Picha, Kathleen S" uniqKey="Picha K" first="Kathleen S." last="Picha">Kathleen S. Picha</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Immunex Corporation, Seattle, WA 98101</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Cosman, David" sort="Cosman, David" uniqKey="Cosman D" first="David" last="Cosman">David Cosman</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Immunex Corporation, Seattle, WA 98101</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Tushinski, Robert J" sort="Tushinski, Robert J" uniqKey="Tushinski R" first="Robert J." last="Tushinski">Robert J. Tushinski</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Immunex Corporation, Seattle, WA 98101</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Gillis, Steven" sort="Gillis, Steven" uniqKey="Gillis S" first="Steven" last="Gillis">Steven Gillis</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Immunex Corporation, Seattle, WA 98101</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Baker, Paul E" sort="Baker, Paul E" uniqKey="Baker P" first="Paul E." last="Baker">Paul E. Baker</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Immunex Corporation, Seattle, WA 98101</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Immunology</title><title level="j" type="abbrev">MIMM</title><idno type="ISSN">0161-5890</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1988">1988</date><biblScope unit="volume">25</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="843">843</biblScope><biblScope unit="page" to="850">850</biblScope></imprint><idno type="ISSN">0161-5890</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0161-5890</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid sequence</term><term>Amino acid sequence homology</term><term>Amino acid sequences</term><term>Amino acid signal sequence</term><term>Amino acids</term><term>Amino terminus</term><term>Assay</term><term>Attta sequence motif</term><term>Biological activity</term><term>Bone marrow cells</term><term>Bovine</term><term>Bovine assay</term><term>Bovine bone marrow proliferation assay</term><term>Bovine cdna</term><term>Bovine cdna clone</term><term>Bovine genomic</term><term>Bovine probe</term><term>Cdna</term><term>Cdna library</term><term>Cell line</term><term>Coding region</term><term>Cosman</term><term>Culture supernatants</term><term>Gasson</term><term>Genomic</term><term>Grabstein</term><term>Granulocyte</term><term>Human cdna probe</term><term>Human factor</term><term>Human probe</term><term>Infectious agents</term><term>Mammalian expression vector</term><term>Marrow</term><term>Mature cells</term><term>Mature protein</term><term>Metcalf</term><term>Mrna</term><term>Murine</term><term>Northern blot analysis</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Phorbol myristate acetate</term><term>Plasmid</term><term>Potential sites</term><term>Previous studies</term><term>Recombinant</term><term>Recombinant murine</term><term>Restriction endonucleases</term><term>Signal sequences</term><term>Southern blot</term><term>Species specificity</term><term>Supernatant</term><term>Transfected</term><term>Wong</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The lymphokine granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates the growth and differentiation of granulocytes and macrophages from bone marrow progenitors, and regulates biological functions expressed by mature cells of these lineages. In order to isolate a bovine GM-CSF cDNA, a cDNA library, generated from the BT2 bovine T cell line, was screened with a human GM-CSF cDNA probe. A cDNA clone was isolated with an insert of 783 bp, that would encode a protein of 143 amino acids, with a predicted mol. wt of 16,160. Bovine GM-CSF exhibits a high degree of sequence homology with mouse and human GM-CSF at both the nucleotide and amino acid levels. Comparison of GM-CSF amino acid sequences from the three species indicates that the bovine GM-CSF precursor contains a putative 17 amino acid signal sequence, cleavage of which would yield a 14,250 mol. wt protein. The cDNA was inserted into a mammalian expression vector and transfected into COS-7 monkey kidney cells. Biologically active bovine GM-CSF was secreted as judged by a bovine bone marrow proliferation assay. Bovine GM-CSF was weakly active in both human and mouse bone marrow proliferation assays. In contrast, human GM-CSF was weakly active on bovine but not murine mouse bone marrow cells and mouse GM-CSF was only active on murine bone marrow cells.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Maliszewski, Charles R" sort="Maliszewski, Charles R" uniqKey="Maliszewski C" first="Charles R." last="Maliszewski">Charles R. Maliszewski</name></region><name sortKey="Baker, Paul E" sort="Baker, Paul E" uniqKey="Baker P" first="Paul E." last="Baker">Paul E. Baker</name><name sortKey="Cerretti, Douglas Pat" sort="Cerretti, Douglas Pat" uniqKey="Cerretti D" first="Douglas Pat" last="Cerretti">Douglas Pat Cerretti</name><name sortKey="Cosman, David" sort="Cosman, David" uniqKey="Cosman D" first="David" last="Cosman">David Cosman</name><name sortKey="Gillis, Steven" sort="Gillis, Steven" uniqKey="Gillis S" first="Steven" last="Gillis">Steven Gillis</name><name sortKey="Picha, Kathleen S" sort="Picha, Kathleen S" uniqKey="Picha K" first="Kathleen S." last="Picha">Kathleen S. Picha</name><name sortKey="Schoenborn, Michael A" sort="Schoenborn, Michael A" uniqKey="Schoenborn M" first="Michael A." last="Schoenborn">Michael A. Schoenborn</name><name sortKey="Tushinski, Robert J" sort="Tushinski, Robert J" uniqKey="Tushinski R" first="Robert J." last="Tushinski">Robert J. Tushinski</name><name sortKey="Wignall, Janis M" sort="Wignall, Janis M" uniqKey="Wignall J" first="Janis M." last="Wignall">Janis M. Wignall</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003218 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003218 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:658059190C2A84658067CF4EECD00BE248EE47DE
|texte= Bovine GM-CSF: Molecular cloning and biological activity of the recombinant protein
}}
| This area was generated with Dilib version V0.6.33. |  |